Demethoxycurcumin inhibits the growth of human lung cancer cells by targeting of PI3K/AKT/m-TOR signalling pathway, induction of apoptosis and inhibition of cell migration and invasion


 Purpose: To determine the antitumor effect of demethoxycurcumin on lung cancer cells, as well its PI3K/AKT/m-TOR signalling, cellular apoptosis, cell migration and invasion.
 Methods: Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, while AO/EB annexin V/PI staining assays were used for apoptosis analysis in demethoxycurcumin-treated A-549 cells. Transwell chamber assay to invasion The expression levels signalling apoptosis-associated proteins cells post- treatment by Western blotting assay.
 Results: Demethoxycurcumin markedly inhibited proliferation a dose- time- reliant fashion. antiproliferative occurred via stimulation apoptosis. Bax, Caspase-3 Caspase-9 increased significantly, Bcl-2 significantly decreased post-demethoxycurcumin treatment. substantially blocked pathway these cells.
 Conclusion: induces anticancer effects targeting PI3K/AKT/mTOR pathway. It inhibits Thus, it is promising anti-lung agent.